25. Prevalence and antibiotics resistance pattern of extended spectrum β–lactamases Escherichia coli strains isolated from chickens

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Ali Zaman, Shakeeb Ullah Syeda Nayab Batool Rizvi Kamran Safdar, Khalid Muhammad Khalid Muhammad, Asif Javaid Umer Khitab Muhammad Inam Malik, Niamat Ullah, Muhammad Shuaib Khan

Abstract

Current work was planned to demonstrate the antimicrobial resistance pattern and prevalence of extended spectrum β-lactamase (ESBL)-producing Escherichia coli and its subtypes from chicken. Antimicrobial resistance of E. coli strains was conducted by Disc Diffusion while ESBL strains were detected by Double Disc Synergy methods. The lower prevalence of the ESBLs strains from the blood samples of healthy chickens (12%) as compared to intestinal contents (13%). The most prevalent ESBLs strains was determined the inducible cephalosporinases bush functional gene 2e (5-20%) followed by carbapenamases (4-10%). The least resistance of E.coli isolates was observed against quinolone group (30-56%)  from blood samples of healthy broilers as compared to cephalosporin group (78-86 %)while intermediate resistance was noted against metallo β-lactam (imipenem and carbapenems; 48%).  Higher resistance was recorded against amoxiclave (68%). Minimum resistance of the E.coli isolates showed against ciprofloxacin (30%) while against cepharadine (100%). Higher resistance was observed against cephalosporin from 78-100%.  Furthermore, the E. coli isolates from birds resisted to metallo β-lactam antimicrobials (imipenem, 45% and carbapenems, 47%). In general, the lowest resistance (40%) was recorded against ciprofloxacin and ofloxacine but 89 and 100% resistance was observed against cephtrioxone and cepharadine, respectively. Furthermore, the quinolone group of the antimicrobial agents was noted as least active against the isolates. Higher prevalence of ESBLs isolates was determined from the feces (51%) on the ground followed by the intestinal contents of diseased chickens (49%).  It was concluded that ESBLs strains of E. coli in bird found highly resistant to majority of antibiotics against them.


Keywords: Birds; Cephalosporinases; Carbapenamases; Disc diffusion; ESBLs strains


http://dx.doi.org/10.19045/bspab.2018.70025

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