Pure and Applied Biology (PAB)

Pure and Applied Biology (PAB) is an online, peer-reviewed, open-access official scientific journal of Bolan Society for Pure and Applied Biology (BSPAB).

PAB is devoted to publish selected papers of latest advancement dealing with original research in Pure and Applied Biology. It is published quarterly by Bolan Society for Pure and Applied Biology (BSPAB) in association with Department of Botany, University of Balochistan, Quetta.

The aim of the PAB journal is to offer a platform for scientists and academicians all over the world to promote, share and discuss various new issues and developments in different areas of Biology.

Pure and Applied Biology (PAB) is HEC RECOGNIZED National Research Journal w.e.f. October 01, 2024 To December 31, 2025

June-2025 Issue ( Vol-14, Issue-2) is now available. Submit your latest Research/Review articles for the Next issue

2017-06-17

June-2025 Issue ( Vol-14, Issue-2) is now available. Submit your latest Research/Review articles for the Next issue

Vol 14 No 2: June-2025

Published: 2025-06-07

Comprehensive analysis of phytochemical composition. Quantitative phenolic profiling by HPLC and assessment of antioxidant activity in Bassia indica

Muhammad Rashid, Shafique Ur Rehman, Saleha Ashfaq, Arifa Siddique, Sajida Afzal, Mansoor Ahmad, Siraj Khan, Syed Saad Shah

82-93

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Optimizing wheat yield through seed rate and planting method interactions: A study for enhanced crop productivity and food security

Shoaib Zawar, Muhammad Waqas Yonas, Muhammad Mujahid Akbar

94-105

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Impact of MCCP infections on production and economics of small ruminants in KPK, Pakistan

Haider Ali Yousafzai, Shahid Hussain Abro, Abdul Sajid, Rani Abro, Dildar Hussain Kalhoro, Hakimzadi Wagan, Abdul Rafi Bhutto

106-114

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Studies on the length weight relationship of Labeo rohita Fish from Rohri canal near Daleel Dero Sakrand Sindh Pakistan

Jamshed Ali, Shaista Jalbani

115-118

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Estimation of interaction between sucking insect/pests of cotton crop and their predator

Azam Ali, Mujahid Niaz Akhtar, Ahmad Ali, Amjad Farooq

119-124

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Biodegradation of COVID-19 face masks using soil microorganisms; Innovative approach towards applied and environmental microbiology

Sarah Zafar, Kashmala Zainab, Fozia Humayon, Taqdees Malik, Marium Zia

125-138

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Enhancing the nutritional value of Splenda (Setaria sphacelata) from a biochemical perspective

Ikram Ullah, Sibghat Ullah, Muhammad Irshad, Naeem Ullah, Zahid Ullah, Ihsan Ullah, Zabit Ullah, Sajjad Khan, Hanif Ur Rahman

139-147

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Host preference study of fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tepridiea) and parasitism potential of Diachasimimorpha longicaudata on different hosts under laboratory condition

Muhammad Amin, Amani Mulk Khan, Muhammad Salim, Imtiaz Ul Haq, Muhammad Salim, Imtiaz Ul Haq, Hamad Akhtar, Shakir Amjad, Fahim Ullah

148-158

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Efficacy of Bacillus spp. and Trichoderma harzianum against bacterial wilt of tomato

Navish, Huma Shah, Muhamamad Inam ul Haq Shahzada Sohail Ijaz, Muhammad Usman Raja

187-196

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Ethnobotanical study of village Thanda Paani in Islamabad, Pakistan

Irfan Mumtaz, Usman Ahmad, Rahmat Wali, Jabar Zaman Khan Khattak

197-205

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Analysis of antibiotic residue presence in poultry meat through microbiological assessment

Muhammad Usama Uzair, Muhammad Afan, Fareeha Imran, Saira Saeed, Muhammad Ramzan, Khadija Tul Kubra, Muhammad Sikandar

206-223

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Identification and pathogenicity of Fusarium species isolated from maize seeds collected from the district Sialkot

Andliba Batool, Brian Gagosh Nayyar, Akasha Naseer, Ayesha Sarwar, Noshaba Olikh, Wajiha Seerat

249-258

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Evaluation of yield potential and agronomic traits of new wheat varieties under agro- ecological conditions of Bannu Division (Khyber Pakhtunkhwa)

Abdul Quddoos, Khalid Mahmood, Muhammad Farooq, Muhammad Ali, Alamgir Khan, Noman Khan

259-265

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Quantitative traits analysis and kinship relationship of indigenous chicken of Sindh, Pakistan

Rameez Raja Kaleri, Raza Ali Mangi, Ghulam Mustafa Solangi, Habibullah Janyaro, Zainab Lanjar, Muhammad Anees Memon, Abdul Jabbar Imran Ahmed, Farooque Ahmed Khushk, Tamseel Saleem, Imtiaz Ahmed Cheema, Muhammad Hashim Baloch, Naqeeb Ur Rehman

266-271

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Impact of different nitrogen levels on maize (Zea mays L.) growth and yield

Asif Ali Kaleri, Muhammad Mithal Lund, Danish Manzoor, Muhammad Sadiq, Salman Adil, Anam Naz, Abid Hussain, Maryum Bilal, Zubair Ahmed Pirzada, Mansab Ali, Moaiz Ur Rehman, Amna Sarfraz

272-282

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In-vitro bioassay of organic nematicides against Root-knot nematode, Meloidogyne incognita (Kofaid & White). Chitwood

Sarir Ahmad, Aneeha Amin, Abdul Haseeb Sumbal Bibi, Muhammad Ilyas Khan, Aimal Khan, Muhammad Mansoor ul Haq,, Salman Khan

283-296

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Effect of different weed management practices on growth and yield of wheat (Triticum aestivum L.)

Ali Raza Gadahi, Ghulam Mustafa Laghari, Asif Ali Kaleri, Danish Manzoor, Afshan Afzal, Ali Raza Mahar, Ameer Jan, Shah Zeb Khan, Mudasir Ali Soomro, Saif Ullah, Amjad Ali Sodhro, Ali Abbas Kaleri

297-303

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Antimicrobial resistance of bacteria isolated from the nasal cavity of camels in district Hyderabad, Sindh

Muhammad Luqman, Dildar Hussain Kalhoro, Shahid Hussain Abro, Hasina Baloch, Muhammad Saleem Kalhoro, Waheed Ali Kalhoro, Hassan Ali, Asad Ullah Marri, Fateh Muhammad Gad, Abdul Suboor Ansari, Israr Hussain Koondhar

304-310

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Exploring the impacts of combining ability of rapeseed (Brassica napus L.) cultivars for agro-morphological characters in water deficit environments

Sajid Hussain Rao, Saima Bano, Abdul Wahid Baloch, Muhammad Arsal, Hajira Imran, Rabab Akram, Wajid Ali Jatoi, Asadullah ,

311-326

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Heavy metals assessment in camel meat, beef and mutton through atomic absorption spectrophotometer in Quetta

Faiza Khan, Samiullah, Naqeebullah Khan, Attiq-Ur-Rehman, Abdul Hakeem

327-331

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Effects of zinc solubilizing bacteria on the growth dynamics of wheat germplasm

Sundus Malik, Aqib Iqbal, Iqbal Munir, Muhammad Arif

332-340

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Frequency of uropathogens and isolation of extended-spectrum beta lactamases (ESBLs) secreting uropathogenic Escherichia coli (UPEC) at Hyderabad region

Naveen Qadeer, Shagufta Jabeen, Beenish Khanzada, Abdul Saleem Qadeer, Jawaid mian Larik, Mahnoor Nisar

341-351

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Impact of zinc levels on growth and seed yield of variety Dadu Maize-1 under the agro climatic conditions of Tandojam Sindh-Pakistan

Haresh Kumar, Muhammad Mahamood-ur-Rahm Jamro, Zulfiqar Ali Abbasi, Muhammad Nawaz Kandhro, Amanullah Tunio

352-361

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Some studies of the renal function in Type-I vs Type-II Diabetics from hospitals District Multan (Punjab) Pakistan

Muhammad Zafar, Muhammad Muzammil Malik Samrah Masud, Muhammad Hashir Latif, Muhammad Taqadas Sajjad, Muhammad Wajid, Misbah Abrish, Tuba Khan, Asad Mumtaz Usama Mumtaz, Sajid Ali, Rabia Abdur Rehman, Maryam Khalil

362-365

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Rapid massclonal regeneration of chickpea (Cicer arietinum L.) through callus induction

Sayyed Zakir Hussain Shah, Shahla Karim Baloch, Moharam Ali, Nighat Seema Soomro, Allah Jurio Khaskheli, Mohsin Ali Khaskheli, Zohra Fatima Memon Bilqees Magsi, Amar Meghwar, Israr Ahmed, Asghar Ali Rajpar

366-375

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Evaluation of mealworm meal as an alternative to Soyabean meal in the diet for white leghorn layers

Irfan Ullah, Sarzamin Khan, Sarir Ahmad, Sumbal Bibi, Muhammad Ilyas Khan, Aneeha Amin, Muhammad Mansoor ul Haq, Salman Khan, Aimal Khan

376-384

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Influence of Humic acid and Potassium levels on the performance of courgette crop

Atif Ur Rehman, Muhammad Noman Khan, Naila Gul, Komal Aslam, Saira Sattar, Muhammad Sulaiman, Waqas Ali, Nasar Khan, Eisa Khan

385-401

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Efficiency of Episyrphus balteatus larvae against Myzus persicae An: In-depth laboratory analysis

Kamal Khan Abro, Naheed Baloch, Farooque Ali Hattar

402-411

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The inheritance studies in different quantitative and qualitative traits in Soybean (Glycine max L.)

Kamran Khan Kaleri, Shabana Memon, Asif Ali Kaleri, Shazia Yasin, Danish Manzoor, Hafsa Munir, Ayesha Sadiqa, Saif Ullah, Mahmood Ur Rehman, Amjad Khan, Sikandar Ali

412-420

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Status of market milk sold for human consumption in Sakrand city of Sindh province

Muhammad Arslan Yousaf Rehan, Abdul Samad Mangsi, Faiz Muhammad Khand Shakeel Ahmed Shar, Muhammad Usama, Kashif Inayat, Muqadas Lund, Muhammad Abdul Saboor

421-432

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Nutritional assessment of teenage girls at Tando Allahyar, Sindh, Pakistan

Afroz un Nisa, Afsheen Mushtaque Shah, Beenish Khanzada, Shagufta Jabeen, Jawaid Mian Larik

433-439

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Study on the analysis of yield and fiber quality characteristics of upland cotton (Gossypium hirsutum L.)

Rajab Ali Kaleri, Naila Gandahi, Mohsin Zulqarnain Hussain Abbasi, Shahla Karim Rukhsar Samoon, Tariq Hussain Majeedano, Nabeela Rashid, Aweesha Bughio, Soohanro Khan Kaleri, Farman Ali Kaleri Naeem Ahmed Kaleri, Sarmad Ali Kaleri

440-445

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Chemical composition, anti-bacterial and anti-fungal activity of essential oil of Nannorrhops ritchiana growing wild in district Kech, Balochistan

Mahnaz Kareem, Ashif Sajjad, Farzana Anwar, Abdul Saboor, Fouzia Noreen Kulsoom Baloch, Fareeda Marri, Ayeesha Lal, Muhammad Nazish

446-454

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Molecular detection and cloning of bacterial thermophilic cellulase gene isolated from hot spring of Gilgit Baltistan

Mushahid Khan, Nawab Ali, Muhammad Nughman, Saadat Khan, Kainat Ali Zulqarnain, Ayesha Gul, Faheem Ullah, Qasim Khan, Muhammad Haq Nawaz

455-467

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Impact of various organic amendments on soil microbial biomass and enzymes activities under different cropping patterns; an incubation study

Rehmat Ullah, Zafar Abbas, Sobia Noor, Muhammad Bilal, Fareeha Habib, Farhat Bashir

479-498

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Alginic acid level, its physical properties and biochemical composition of three selected brown seaweeds (Colpomenia sinuosa, Dictyota dichotoma and Iyengaria stellate) of Karachi coast, Arabian Sea

Fozia Khan Siyal, Rashida Qari, Muhammad Tahira Jatt

499-506

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Formulation and upscaling of herbal shampoos by Aloe vera L. and Camelia sinensis L. powders

Ayesha Fatima, Uzma Hanif, Romana Aziz Adeel Mustafa, Hafsa Yaqoob, Rabia Shafique, Aqsa Maqsood, Hifza Hanif

507-514

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Study on effect of grouped and individual housing system on growth performance and feeding behaviour of post weaned buffalo calves

Agha Raza Ali Khan, Huma Rizwana, Muhammad Farooque Hassan, Habibullah Janyaro, Imtiaz Ahmed Cheema, Reema Bughio, Muhammad Anees Memon, Imran Ahmed, Awais Memon, Abdul Jabbar Mudasar Ahmed Khosa, Shoaib Akhlaq, Rameez Raja Muhammad Kaleri

515-523

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Genetic diversity for yield and fiber traits in upland cotton (Gossypium hirsutum L.) genotypes

Rajab Ali Kaleri, Younis Malik, Tariq Majidano, Mohsin Zulqarnain Hussain Abbasi, Saleem Ejaz, Ghulam Hassan Bahalkani, Nabila Maryam Muhammad Ubaidullah Aleem, Zuhaib Saleem Asma Kaleri, Benazir Chutto, Farman Ali Kaleri, Kamran Khan Kaleri, Muhammad Irfan Ali Kaleri

524-530

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Effect of urea foliar application at tillering and booting stages on yield of wheat (Triticum aestivum L.) under Agro climatic condition of Tandojam

Imtiaz Ali Tunio, Shazia Yasin, Tanveer Hussin Tunio, Aslam Panhwar, Jan Muhammad Keerio, Suresh Kumar

543-550

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Soil phosphorus evaluation and mapping of onion growing areas of tehsil Nall, Balochistan

Atta Muhammad, Sher Azam, Muhammad Ibrahim Mengal, Abdul Nabi Muhammad Akbar, Muhammad Luqman Khan Bazai, Abdul Aziz, Irfan Qadir, Zahoor Ahmed, Muhammad Aamir Tariq, Shabeer Ahmed, Behroz Jan Jamaldini, Abdul Muhammad Raziq, Adam Khan

551-575

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Neurobehavioral deficits of AlCl3 attenuated by administration of Turmeric supplementation in rats

Shoaib Ahmed, Irfan Sajid, Lubna Anis, Hafsah Khan, Syed Ikhlaq Hussain, Wahaj Uddin Qureshi

576-584

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Assessing the performance of maize genotypes and nitrogen levels on agronomic traits and phytochemical composition of corn

Inayat Ullah, Khalid Naveed, Arbaz Hassan, Saman Khalid, Muhammad Sheraz Muhammad Ibrar Hassan, Arshad Iqbal, Zehnoor Ahmad, Rafaquat, Malik Muhammad Faizan Shoukat, Abid Mehmood

585-598

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Impact of different sowing dates on yield and agronomic traits of wheat under arid and semi-arid conditions of Bannu Division

Abdul Quddoos, Khalid Mahmood, Muhammad Ali, Muhammad Farooq, Alamgir Khan, Noman Khan, Bashir Ullah

599-606

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Characterization and distribution of rainfed soils of district Chakwal for sustainable agricultural production

Rizwan Latif, Abdul Latif, Muhammad Arsalan, Madeeha Khan, Rehmat Ullah, Muhammad Bilal, Azhar Mahmood Aulakh, Sabir Hussain Shah, Muhammad Muhammad Abdullah

607-615

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Understanding the responses of two wheat genotypes to individual and combined drought and heat stress during reproductive and grain filling stages

Lubna Naz, Muhammad Sumayya Inayat

616-629

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Effect of watering frequency on milk parameters, feed and water intake in Achai-Jersy cross cattle

Zahid Ullah, Khisrao Kalim, Sibghat Ullah, Muhammad Irshad, Naeem Ullah Fakhar uz Zaman, Zabit Ullah, Sajjad Khan, Muhammad Ikram Ullah

630-636

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Effect of linseed oil on histopathology of reproductive organs of Rabbit

Mishal Mubasher, Muhammad Razia Iqbal

637-649

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To determine the combining ability effects of mustard (Brassica juncea L.) genotypes for morphological and physiological characters in water deficit condition

Saima Bano, Sajid Hussain Rao, Abdul Wahid Baloch, Rabab Akram, Hajra Imran Muhammad Arsal, Asadullah, Siraj Ahmad Muhammad Channa

650-663

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Effect of various concentrations of pomegranate peel waste on the growth performance and nutrient digestibility of broilers

Shahzad Ahmed Unar, Azizullah Memon, Dildar Hussain Kalhoro, Waheed Ali Kalhoro, Faisal Ali Samo, Israr Ahmed Kondhar, Jahanzaib Khaliq Fayaz Ali Laghari, Muhammad Ismail Brohi, Fahad Hussain Tunio, Shua Namood, Muhammad Zulqarnain Unar

664-674

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Pharmacognostic and phytochemical evaluation of Capparis cartilaginea Decne., leaf: A medicinal plant

Uzma Munir, Ishrat Jamil, Shazia Muhammad Mansuri, Anjum Perveen

675-683

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Assessing the vulnerability and economic burden of lumpy skin disease on indigenous and exotic cattle in Sindh, Pakistan

Muhammad Awais Memon, Shahid Hussain Abro, Dildar Hussain Kalhoro, Nazeer Hussain Kalhoro, Rani Abro

684-691

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Genetic diversity exploration in (Capsicum annuum L.) sweet pepper germplasm through chemical and morphological characterization

Nadra Khan, Kashif Khaqan, Muhammad Muhammad Jamil Ahmed

692-709

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Enzymatic responses in Oryzaephilus surinamensis (Coleoptera: Silvanidea) to plant extracts, entomopathogenic fungi and bacterial agents: Implications for pest control

Usama Afzal, Farid Asif Shaheen, Qurban Ali, Asad Aslam, Muhammad Shehzad, Humaira Malik, Muhammad Kamil Malik, Muhammad Faheem Akhtar, Muhammad Bilal Bin Iqbal, Muhammad Jawad Saleem, Najuf Awais Anjum

724-733

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Comparative biochemical analysis and profiling of sucrose-metabolizing enzyme activity in female and hermaphrodite papaya fruits at different phenological stages

Zunaira Riaz, Ishrat Jamil, Faisal Mehdi, Shagufta Sahar, Nabeela Afzal, Saddia Galani

734-746

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Fungal immunomodulatory proteins (FIPs) from Ganoderma lucidum: Unveiling their immunomodulatory mechanisms and potential for cancer therapy

Hafsa Khalid, Zunaira Azam, Marya Saadullah Khan, Dua-E-Jamila Khurrum, Afifa Laraib, Muhammad Bilal, Rimsha Haqqi

159-186

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Extraction techniques and purification methods: Precision tools for pure innovation

Maria Noor, Nisa Basharat, Muhammad Asad Riaz Hussain, Muhammad Sheraz Arif, Ghulam Qadir, Soban Khalid, Sadam Hussein, Aliha Asif, Faisal Ul Rehman, Malik Faizan Shaukat, Abid Mehmood

224-248

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A mini review on genome editing via CRISPR-CAS system to enhance agronomic traits of wheat and tomato against environmental stresses

Muhammad Danyal Mustafa, Sumaira Mazhar, Adeeba Syed, Umair Ali

468-478

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A comprehensive review on resistant starch, its types, sources, application and health benefits

Riaz Hussain, Maria Noor, Soban Khalid Nisa Basharat, Hassan Raza Muhammad Sheraz Arif, Atika Iffat, Malik Faizan Shoukat, Rafaquat Khalid Naveed, Hajra Zeb Abid Mehmood

531-542

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Influence of pre harvest ethephon application on various physical, physiological and biochemical attributes of agricultural crops: A-review

Muhammad Noman Khan, Naila Gul, Komal Aslam, Muhammad Irshad, Anjum, Sajid Siddique, Dawood, Mehwish, Salman Khan, Muhammad Fazalullah

710-723

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Open Access Journal

Open Access is a publication model that enables the dissemination of research findings to the global community without restriction. Thus, all articles published under open access can be accessed by anyone with internet connection.

Creative Common License

The articles are published in PAB as an open access articles distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).