TY - JOUR AU - Muhammad Nisar Anjum, Faiz Ahmad Joyia, Ghulam Mustafa, Rubab Zahra, Saddam Munawar, AU - Khan, Muhammad Sarwar PY - 2019 TI - 80. Exploiting shoot tips as an efficient explant for in vitro regeneration of cucumber (Cucumis sativus L.) JF - Pure and Applied Biology (PAB); Vol 8 No 2: June-2019 KW - N2 - A proficient and speedy in vitro regeneration is a prerequisite for genetic modification of plants. The described protocol presents an efficient and practicable procedure for regeneration and multiple shoots induction by using shoot tips as explants for cucumber ( Cucumis sativus L.). First, aseptic seedlings were established and shoot tips were excised from those seedlings. The cucumber seedlings used after 7 days of germination were found to be the best source of explants. Theses shoot tips were placed on MS medium supplemented with different concentrations of Benzyl amino purine (BAP) (0.5-2.5mg/L). It was observed that MS medium augmented with 1.5mg/L BAP resulted in maximum shoots formation. By increasing BAP beyond 1.5mg/L, rate of shoots formation was decreased. For rooting purpose, Indole Butyric Acid (IBA) (0.5-2.5mg/L) was used. Shoots greater than 2cm in size were shifted to rooting medium. Best roots development took place on MS medium having 1mg/L IBA. The plantlets with well-developed roots were shifted to peat moss containing pots and gradually acclimated to soil. This in vitro regeneration protocol can be used for genetic transformation of cucumber in future. Keywords : BAP; Cucumber; IBA; In vitro regeneration; Shoot tips http://dx.doi.org/10.19045/bspab.2019.80126 UR - https://thepab.org/index.php/journal/article/view/977