14. Detection of antibiotic resistance genes in Lactobacillus and its role in transferring these genes to Salmonella

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Muhammad Ashraf, Sidra Anam, Faisal Rasheed Anjum, Sultan Ali, Rizwan Aslam

Abstract

Antibiotic resistance is a worldwide issue and becoming problematic due to extensive misuse of antibiotics. The present study was aimed to analyze the role of Lactobacillus in transmission of antibiotic resistance genes (tetM, ermB, sul2) to Slamonella and verification of these genes by quantitative polymerase chain reaction. A total of thirty fecal samples (15 were indigenous and 15 were broilers) were collected and genomic DNA was isolated. Both Salmonella and Lactobacillus were confirmed by polymerase chain reaction. The expression and quantification of antibiotic resistance genes was done by quantitative polymerase chain reaction. The results indicated that there was higher expression of antibiotic resistance genes in Lactobacillus isolated from broiler chicken than the isolates obtained from indigenous birds indicating Lactobacillus as a reservoir of antibiotic resistance genes. But the role in transferring these genes to Salmonella was found to be non-significant. In conclusion, the excessive use of animal growth promoters in poultry assists in acquisition of antibiotic resistance by Lactobacillus and contributes in spread of antibiotic resistant determinants.


Keywords: Antibiotics; Determinants; Quantitative polymerase chain reaction


http://dx.doi.org/10.19045/bspab.2019.80145

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