Antibacterial and antibiofilm potential of leaves extracts of Mirabilis jalapa L. and Ajuga bracteosa wall. against Pseudomonas aeruginosa
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Abstract
In the Present study the antibacterial and antibiofilm potential of Mirabilis jalapa and Ajuga bracteosa crude methanolic extracts of leaves against P1, P2 and P3 clinical strains of Pseudomonas aeruginosa was explored. Antibacterial activity was investigated by agar well diffusion method and the antibiofilm potential was determined by Anti-Pellicle assay and exopolysaccharide inhibition (EI) by Congo red (CR) assay. In antibacterial assay, M. jalapa extract showed good antibacterial activity against P. aeruginosa with maximum 13.5 mm inhibition zone (ZI) against P2 strain and minimum 12.8 mm ZI against P3 strain of P. aeruginosa as compared to A. bracteosa which showed a maximum 13.4 mm ZI and a minimum of 12.7 mm against both P2 and P3 strains of P. aeruginosa, respectively. Both M. Jalapa and A. bracteosa extracts showed moderate (+++) and weak (++++) effect on pellicle inhibition against the tested strains of P. aeruginosa. In CR assay, a moderate antibiofilm effect was recorded on EI for both plants. Based on the results it was concluded that methanol extracts of M. jalapa and A. bracteosa possessed good antibacterial activity and moderate antibiofilm potential against P. aeruginosa.
Keywords: Agar well diffusion assay; Ajuga bracteosa; Mirabilis jalapa; Natural products; Opportunistic pathogen