2. A review on nanopore sequencing technology, its applications and challenges

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Mirza Jawad Ul Hasnain Bakhtawar Afzal Tehreem Anwar, Muhammad Tariq Pervez Tanveer Hussain


In this review, we describe another age of sequencing ‘Nanopore Sequencing technology” which is rapidly growing to fulfill the gap in advancements, which hold the capacity for significantly larger perused read lengths, less time consuming and lower by expense. Nanopore-based sequencers, as the fourth-generation DNA sequencing technology, have the potential to quickly and reliably sequence the entire human genome for less than $1000, and possibly for even less than $100.The need has never been more prominent for progressive advances that convey time efficient, reasonable and exact genome data. This quest has led to the advancements in the field of Next Generation Sequencing (NGS). The economical production of huge volumes of sequencing information is the essential advantage over traditional techniques. The preliminary methods of sequencing namely Sanger sequencing and Second Generation Sequencing provide the basis to the propelling and surprising number of logical advances. However, the evolving genomics and specifically the sequences sciences provide prolific basis for extra development in this space of research. We hereby ponder upon the broad range of Nano-pore sequencing techniques along with their applications in the emerging era of genomics and its advancement, also the word goes towards the importance of Nano-pore sequencing advancements in the emerging markets of sequencing like that of Pakistan.

Keywords: Genome; Nano-pore; Next-Generation Sequencing; Sequence analysis; Technology



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