35. In vitro shoots multiplication from nodal explants of Sesame (Sesamum indicum L.)

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Muhammad Asad, Nadeem Ahmed Amir Sohail Tanveer Burni, Fazal Hadi, Rehman Ali Muhammad Nauman Khan Abdullah Aziz, Abbas Muhammad


An effective micro propagation system has been developed efficiently through an enriched culture system for Sesamum indicum L. used as a medicinal plant and spicy crop significant from a nutritional point of view. The multiplication of shoot was obtained on Murashige and Skoog medium supplemented with 15 g l-1 sucrose, 8 g l-1 agar and fortified with benzyl amino purine (BAP) in rabies of 0.1 -1 mg / l. The maximum number of young shoots was appeared on MS medium supplemented with 0.5 mg / l benzyl amino purine (BAP). Multiple combinations of harmonics were used for multiple inductions. Developed micro-shoots were shifted to culture medium supplemented with IAA (1.0 mg / l) for efficient rooting. For hardening newly developed shoots were carried out and 71% were survived effectively. This system can be successfully applied for mass propagation of Sesamum indicum.    

Keywords: BAP; Medium MS; Micro propagation; Multiple shoots; Sesame



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