35. In vitro shoots multiplication from nodal explants of Sesame (Sesamum indicum L.)
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Abstract
An effective micro propagation system has been developed efficiently through an enriched culture system for Sesamum indicum L. used as a medicinal plant and spicy crop significant from a nutritional point of view. The multiplication of shoot was obtained on Murashige and Skoog medium supplemented with 15 g l-1 sucrose, 8 g l-1 agar and fortified with benzyl amino purine (BAP) in rabies of 0.1 -1 mg / l. The maximum number of young shoots was appeared on MS medium supplemented with 0.5 mg / l benzyl amino purine (BAP). Multiple combinations of harmonics were used for multiple inductions. Developed micro-shoots were shifted to culture medium supplemented with IAA (1.0 mg / l) for efficient rooting. For hardening newly developed shoots were carried out and 71% were survived effectively. This system can be successfully applied for mass propagation of Sesamum indicum.
Keywords: BAP; Medium MS; Micro propagation; Multiple shoots; Sesame
http://dx.doi.org/10.19045/bspab.2020.90035