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An assessment was accomplished, to evaluate the prevalence and incidence of Tylenchus semipenetrans (Cobb.) in district Layyah, in the year 2016. A total of 180 soil and root samples were collected from 60 localities of district Layyah in Pakistan. The analysis revealed that 46.67% of the samples were infested with the nematode. Maximum infestation (61.67%) was recorded in tehsil Chobara followed by Layyah (43.34%) and Karor lal-eesan (31.67%), respectively. In tehsil Chobara nematode in soil ranged from 1,102 to 11,070 per kg of soil and number of females ranged from 274 to 1,202 per gram of root followed by tehsil Layyah and Karor lal-eesan ranged from 918 to 10,430 and 1,018 to 9,854 and females per gram of root ranged from 168 to 857 and 120 to 990 respectively. This information is useful to create awareness about the citrus slow decline disease and for its rapid precautionary measures.
Keywords: Citrus decline; Tylenchus semipenetrans; Soil samples; Population dynamics