111. Relationship among protein oxidation and biochemical parameters of healthy and hepatitis C patients

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Umar Khitab, Ikram Ullah Ali Zaman Haseena Baloch Shakeebullah, Muhammad Shoaib Khan Ambrina Tariq Khalid Muhammad, Naimat ullah, Muhammad Inamullah Malik, Saqib Ali Rustam Muhammad Noman

Abstract

HCV is internationally recognized health issue as it causes chronic liver diseases which are difficult to diagnose with symptoms until it causes sever liver damage which may leads to liver cancer. Oxidative stress caused by hepatitis leads to the damage of the DNA and oxidation of both lipid and protein. In the present study research was conducted to find and correlate hepatitis C with protein oxidation, DNA damage and lipid peroxidation. Samples were collected from 50 suspected individuals including 20 with hepatitis C virus (HCV) and 30 healthy ones. The protein carbonyls quantification was performed by using 2, 4 dinitrophenyle hydrazine assay (2,4 DNPH assay). The results of DNPH assay clearly showed that protein Carbonyl level was much higher than normal controls. Finally, HCV biochemical parameters i.e. cholesterol, triglycerides and complete blood count were compared. The results indicated that biochemical parameters were also significantly changed in patients when compared with control. This project elaborates a new era particularly the chronic HCV patients having severe liver dysfunction, abnormal proteins oxidation and biochemical parameters. On the basis of the current results, we concluded that oxidative stress was involved in abnormal biochemical parameters, protein oxidation and lead to liver damage.


Keywords: Biochemical parameters; HCV; Liver Cancer; Oxidative stress; Protein oxidation


http://dx.doi.org/10.19045/bspab.2020.90122


 

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