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The allelopathic potential of Euphorbia helioscopia L. was investigated against Brassica campestris L. and Triticum aestivum L. in the laboratory. Fresh and dry leaves of Euphorbia helioscopia of 5g, 10g and 15g weight were soaked in 100ml of distilled water. These materials were filtered after 24 and 48 hours. The extract of fresh and dry leaves of Euphorbia helioscopia was applied in 5g, 10g and 15g concentration on Brassica campestris and Triticum aestivum in order to investigate the allelopathic impact on radicle and plumule length and germination percentage after 72hrs of incubation period at 26°C. The results showed that the dry and fresh leaves extract of Euphorbia helioscopia significantly reduced plumule and radicle length of both test species while stimulated the germination percentage in Brassica campestris and Triticum aestivum as compared to the control. The inhibitory effect of dried leaves on the test plant was more conspicuous than the fresh leaves. The plumule length was inhibited more in Brassica campestris as compared to Triticum aestivum by dry leaves and was recorded 95.49% and 61.57% in 24 hours soaking extract at 10g concentration and 94.83% and 54.43% in 48 hours soaking extract at 15g concentration. The radicle length B. campestris as compared to Triticum aestivum was effected more by 24 hours soaking extract and was recorded 93.57% in 10g fresh leaves and 15g dry leaves extract. The germination percentage of Triticum aestivum was stimulated by 24- and 48-hours soaking extract at all concentrations of fresh and dry leaves extract. As compared to Triticum aestivum, Brassica campestris was more effected by all treatments. From the result, it was concluded that the dry and fresh extract of Euphorbia helioscopia negatively affected the growth of Brassica campestris and Triticum aestivum and stimulated the germination of Triticum aestivum which suggests that some allelochemicals might be present in Euphorbia helioscopia.
Keywords: Allelopathy; Brassica campestris; Euphorbia helioscopia; Germination percentage; Inhibitory effect; Plumule length; Radicle length; Triticum aestivum