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The experiment was conducted to estimate the phytochemical and antibacterial activity in Adhatoda vasica during the year 2018. The plant samples were collected from the Khanpur valley in fresh forms and were processed in the Food Science and Technology Laboratory The University of Haripur for further chemical analysis. Antimicrobial activity of Adhatodavasica was tested against various gram positive and gram negative bacterial strains. Different phytochemicals compounds i.e. Alkaloids, Steriods, Flavonoids, Terpenoids, Tannins Extract, Saponins, and Glycosides were investigated in the experiment. Highest antimicrobial activity was recorded in case of gram positive bacteria. Leaf extracts of Adhatodavasica leaves extract would be replacement to synthetic/aritificial antimicrobial agents and needs further studies and clinical trials. The phytochemical screening indicated presence of secondary metabolites such as alkaloid, flavonoids, tannins and phenol in both extracts making it to have antibacterial potentials. Extract showed remarkable activity against the growth of the selected bacteria The presence of these phytochemicals may lead to the conclusion that Adhatodavasica plants can be used as medicine for treating different diseases.
Keywords: Adhatodavasica; Antibacterial activity; Escherichia coli; Phytochemicals; Staphylococcus aureus