1. Antimicrobial activity of ethanolic dye extract of onion scale leaves
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Abstract
The phytochemical composition and pharmacological value of natural dye extracted in ethanol from scale leaves of Allium cepa was investigated. The dye showed the presence of alkaloids (7.10%), flavonoids (0.127%), tannins (0.013%) phenols and (71.6736 mg gallic acid eq. /g). Extract of dye was evaluated against 3 strains of bacterial (Klebsiella pneumonia, Staphylococcus aureus & Acinetobacter baumannii) and 3 strains of fungi (Aspergillus .fumigatus, Aspergillus niger and Aspergillus flavous,). The zone of inhibition against S. aureus was 21 mm, K. pneumoniae was 19 mm and A. baumannii was 18.5 mm. The Maximum anti-fungal activity of dye was reported against A. flavous. The antioxidant activity was done as percent inhibition of DPPH (1, 1-diphenyl-2-picryl-hydrazyl), free radicals. The DPPH radical scavenging activity was observed for dye and was higher at 200 µg/ml. It is concluded that Allium cepa dye could be a potential source of pharmacologically important compounds.
Keywords: Antimicrobial activity; Ethanolic dye; Leaves; Onion