31. Purification and characterization of lipase produced by indigenously isolated bacteria

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Muhammad Aqeel Sultan, Muhammad Javaid Asad, Raja Tahir Mahmood, Majid Mahmood, Sidrah Nazir and Muhammad Basir Shah


Bacteria including Bacillus species often produce extracellular enzymes to degrade substances such as proteins, lipids, and starch.  Glycerol and long-chain fatty acids are hydrolyzed by lipases (triacylglycerol acylhydrolases, EC to generate esters. Bacterial strains were isolated from rotten fruits and vegetables and cultured at 20 to 50 0C with different types of substrates (Olive oil, Mustard oil and Tributryne). Extracellular enzymes produces from bacteria with different physical parameter and also using different substrates. Only two strains, strain 1 and strain 2 show results when provided substrate Olive oil and Mustard oil at pH 7 and at temperature 300C but Tributryne show result at pH 9 and at temperature 400C. Addition of maltose and fructose increased the lipase activity to 28.53U/ mL and 30.17U/mL respectively. The activity enhance to 50.83U/mL by the addition of yeast extract as nitrogen source. Addition of 60% ammonium sulfate results in maximum precipitation with activity 59.46 U/mL. The results concluded that isolated bacterial strains are good producer of lipase enzyme. The purified enzyme could be a good candidate for various industrial applications.

Keywords: Ammonium sulfate precipitation; Bacillus species; Lipase activity; Rotten fruits; Tributryne agar Plate 



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