9. Pharmacognostic study of Walnut (Juglans regia L.) endocarp from Azad Jammu Kashmir (AJK)
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Abstract
The present study explains antimicrobial activity and acute toxicity of walnut (Juglans regia L.) endocarp from Azad Jammu Kashmir (AJK). The walnut endocarp extract tested against fungal species i.e. Aspergillus niger and Penicillium notatum and bacterial species i.e. Staphylococcus aureus and Escherichia coli. Aspergillus niger and Penicillium notatum. at 10, 100 and 1000 µg/ml concentrations showed Diameter Inhibition Zone (DIZ). Diameter Inhibition Zone (DIZ) was maximum against Aspergillus niger in all concentrations as compared to Penicillium notatum. Similarly, Staphylococcus aureus showed higher Diameter Inhibition Zone (DIZ) at 10, 100 and 1000 µg/ml concentration respectively as compared to Escherichia coli Diameter Inhibition Zone (DIZ) at the same concentrations. Acute toxicity of walnut endocarp extract showed the significant result at 100 mg/kg, 200 mg/kg and 400 mg/kg concentrations with 0% mortality. It was concluded that walnut endocarp may be important source of antimicrobial activity and may be used in pharmacognosy.
Keywords: Acute toxicity; AJK; Antimicrobial activity; Pharmacognocy; Walnut