23. Starch hydrolyzing enzyme production from Aspergillus niger EFRL-FC-024 using molasses as carbon source
Main Article Content
Abstract
Amylase is one of the important industrial enzyme due to its application in detergents, food and textile industries. In present study, the production of amylase, a starch hydrolyzing enzyme, was optimized using Aspergillus niger strain EFRL-FC-024 under various conditions i.e. incubation time, carbon source, pH and temperature. Amylase production was recorded as 2.26 U/mL after 96 h of incubation in mineral medium containing glucose as carbon source. In contrast its concentration increased to 2.55 U/mL when molasses was added to mineral medium. However, the highest enzymes titer of 2.97 U/mL was achieved in optimized conditions (1.0% w/v molasses, 96 h, pH 6.0 and temperature 40ºC). Molasses are a cost-effective carbon source for amylase production using Aspergillus niger strain EFRL-FC-024. Thus this enzyme can be produced cost effectively using low cost agro-industrial waste natively like molasses and could be utilized in detergent or textile industry or even for the saccharification of starchy materials i.e. potato waste to produce ethanol and other commodity products.
Keywords: Amylase; Aspergillus niger EFRL-FC-24; Molasses; Starch hydrolyzing