22. Phytochemical analysis and antimicrobial activities of Kochia indica (Wight), plant growing in District Karak Khyber Puhktunkhuwa, Pakistan

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Hameeda Bibi, Manzoor Hussain, Gul Jan, Ghulam Mujtaba shah, Siraj Khan, Ihsan Ullah


The medicinal plants play a key role for the treatment of various types of diseases because of bioactive compounds. Study aims, to investigate the antibacterial potency, phytochemical analysis and also perform gas chromatography and mass spectrometry (GC-MS) for isolations of bioactive compounds of Kochia indica Wight plant. In antibacterial activity the whole plant extracts of Kochia indica Wight, against S. typhi, Escherichia coli, Shigella flexneri, Pseudomonas aeroginosa and Staphylococcus aureus strains at different concentrations. According to the results obtained, the ethanol extract of Kochia indica was 40%-20.37% (15 mg/ml), 13.11%-19.10% (10 mg/ml) and 11.20%-17. 44%, (5mg/ml) concentration over negative control. The best quantitative phytochemical analysis of Kochia indica Wight was performed using extracted optical density (OD) and mass chromatography-mass spectrometry (GC-MS) techniques. Results reveal the presence of important antibacterial and phytochemical compounds predominantly1-Propanol, 2-methyl- (63.27 %), 1,1-Diisobutoxy-isobutane (8.83%), Oleic Acid (7.54%), Cyclohexanone (6.60%), Acetone (3.57%), Hexanoic acid, 2-methylpropyl ester (2.75%), n-Hexadecanoic acid (1.47%), 2-Butenedioic acid (Z)-, dibutyl ester (1.41%), Sebacic acid, ethyl hexyl ester (0.93%), Phytol (1.00%), Octadecanoic acid (0.78%), Cyclopentane carboxylic acid, butyl ester (0.65%), 1-Butanol (0.42%), Propanoic acid, 2-methyl, 2-methylpropyl ester (0.48%), Cyclohexene,1-(1,1-dimethylethoxy) (0.31%). To detect more effective process and compounds to initiate and develop the most effective approaches and methods to reduce the effectiveness of bacteria on environment as well as in human health.

Keywords: Antibacterial activity; GC-MS analysis; Kochia indica Wight; Phytochemical analysis


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