48. Seroprevalence of Brucellosis in cattle of Swat Valley Khyber Pakhtunkhwa Pakistan
Main Article Content
Abstract
Brucellosis has been an emerging, zoonotic disease that is associated with chronic debilitating infections in humans and reproductive failure in domestic animals. The aim of this study was to investigate the seroprevalence of brucellosis in samples collected from cattle in Swat valley based on the Rose Bengal precipitation test (RBPT), milk ring test (MRT), and indirect enzyme-linked immunosorbent assay (i-ELISA for both milk and serum). A total number 0f 310 samples were processed during the study which consists of 200 serum samples and 110 milk samples. The 200 serum samples were collected from male and female cattle (n=11and n=189).The RBPT and i-ELISA (serum) detected antibodies against B.abortus showed 1.587%, 2.12% in females through both tests respectively, and 0.0% in males on both techniques. The milk samples (n=110) detected B.abortus antibodies 0.9%, 1.82% through MRT and i-ELISA (milk) respectively. The comparative efficacy of MRT and i-ELISA (milk) was also analyzed statistically by z-test, the data revealed insignificant results (p= 0.561) also statistical analysis of RBPT and i-ELISA (serum) findings showed insignificant results (p= 0.703) therefore both results are higher than the significant level (0.05).
Keywords: brucellosis; ELISA; Milk ring test; Rose Bengal plate test; Sero-prevalence