9. Efficacy of Allicin against multi-drug resistant Escherichia coli recovered from potable water

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Sehrish Nayab, Sajjad ur Rahman, Sanaullah Sajid, Muhammad Ismail Abbas, Zia ud Din Sindhu, Muhammad Idrees Muhammad Usama Tariq, Rabia Kanwar, Ifra Farzand

Abstract

The increased prevalence of E. coli, especially the multidrug resistant E. coli recovered from drinking water supplies, demands a new substance, allicin that may potentially be effective and decrease the spread of these microorganisms. Allicin is an antibacterial agent derived from garlic to test its efficiency against multi-drug resistant (MDR) E. coli. The presumptive coliforms were isolated followed by confirmation of pathogenic strains from potable water supply samples received during 2018-2019. Isolated E. coli were found resistant against multiple drugs including Amikacin, Ampicillin, Vancomycin, Oxacillin, Fusidic acid, Gentamycin, Oxytetracycline and Moxifloxacin. The methanolic and ethanolic extracts of garlic were prepared and the allicin compounds were analysed quantitatively through HPLC. The standard concentration of E. coli (0.5 McFarland) was maintained to determine the MIC50 through microdilution technique for both allicin extracts separately. The minimum inhibitory concentration (MIC50) of methanolic and ethanolic derivatives of allicin was found at 28.64μg/ml and 7.57μg/ml of crude allicin extracts respectively. Out of three consecutive concentrations of ethanolic allicin extracts in the experimental wash water containing MDR E. coli was capable to clean the water at 8.00 μg/ml in 10 minutes followed by 10.00 μg/ml and 12.00 μg/ml in 5 minutes of treatment. These results suggested the application of ethanolic derivatives of allicin as potential biocide for cleaning water reservoirs particularly against MDR pathogenic Escherichia coli at dairy farms.


Keywords: Antibiotic resistance; Coliform count; Escherichia coli; Ethnomedicine; Herbal plants; MIC


http://dx.doi.org/10.19045/bspab.2022.110050

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