7. Leaf shape characters’ segregation among anther-derived plants from potato clones
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Abstract
Potato is fourth most important crop in the world, and anther culture is used towards its genetic improvements. Anther culture, as a breeding tool, can influence leaf shape related characters of this species, which warrants further studies. Two potato clones were compared through their anther-derived plants based on five characteristics: overall shape of terminal or primary leaflet, shape of leaflet’s tip, shape of leaflet’s bases, amount of leaf anthocyanin, and pubescence. The anther-derived plants of clone 9507-04 had been regenerated from calli/embryos while clone 6028-02 produced plantlets directly from the anthers. Also, 46-94% of anther-derived plants from clone 6028-02 were similar to the parent while only 37-60% of offspring were found to be parental kinds for clone 9507-04. On confirmation of the DNA contents and/or ploidy of anther-derived plants, it was found that potato clones 9507-04 and 6028-02 produced 77% and 100% monoploids, respectively. The variations, in androgenic fitness as well as culture-response among potato anther-donors, may have an important role for ploidy and leaf morphology differences among anther-derived siblings. By identifying these variations among potato clones, the findings in this report have subsequent applications for generating androgenic plants, with the aim of improving light capture, water balance, as well as temperature regulations by the leaves of crop plants.
Keywords: Androgenic response; Microspore regeneration; Progeny ploidy variations