2. Abundance of Multidrug resistant Shigella flexneri in avian environment in District Haripur, Khyberpakhtunkhwa, Pakistan
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Abstract
Seventy-five fecal, water and soil samples were collected from watering sites of wild migratory species in district Haripur, Khyberpakhtunkhwa, Pakistan. The samples included fecal, water and soil samples of the associated environment of wild birds for detection of multi-drug resistant (MDR) Shigella flexneri. The isolation strains were characterized biochemically and 16srRNA sequencing was done to confirm its presence. The antibiotic sensitivity test was conducted against different antibiotics by Kirby-bauer disc diffusion assay. The results obtained indicated that 36% samples harboured S. flexneri and these strains were 100% resistant to 4 antibiotics including Penicillin, Vancomycin, Azetreonam, and Ertapenam used. The isolated strain sequence was submitted to National Center for Biotechnology Information (NCBI) GenBank for accession no. of LC-521305. These findings compel regular surveillance of pollution of multi-drug resistant strains in avian environment.
Keywords: Azetreonam; Ertapenam; Penicillin; Shigella flexneri; Vancomycin