Detection, partial purification and characterization of Bacteriocin from Enterococcus species indigenous to Azad Jammu and Kashmir

Abstract

Study was carried out to isolate, partially purify and characterization of   bacteriocin from enterococcus species indigenous to Azad Jammu and Kashmir.  Isolation of bacteriocin from raw milk of buffalo was carried out by Spread Plate method and identified by biochemical test. Out of total milk samples (N=20) only samples (N=4) were found to be bacteriocin producing. The demonstration of selected strains was confirmed by 16S rRNA sequence of gene. The selected strains identified as Enterococcus faecium CGMCC 1.2136^T, Macrococcus caseolyticus ATCC 13548^T, Streptococcus gallolyticus sub species macedonicus ACA-DC 206^T and Enterococcus faecium CGMCC 1.2136^T. To  partially  purify the  bacteriocin obtained from Enterococcus species. Ammonium sulphate precipitation method was used after the partial purification of bacteriocin to show its activity as per related enterococal strais and  against identified microorganisms of hospital. High temperature stability (up to 100°C)  was observed. Bacteriocin activity was also seen on larger range  of pH (3 to 9). Treatment with proteolytic enzyme proved the protenecious nature, as the inhibitory property of bacteriocin was completely lost after being exposed to enzyme proteinase K.

Keywords: Bacteriocin; Enterococcus; Identification; Purification

http://dx.doi.org/10.19045/bspab.2023.120157