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Determination of antioxidant activity of the two-endemic plants of northern Balochistan Haloxylon griffithii of Chenopodiaceae family and Convolvulus leiocalycinus of Convolvulaceae family is described for the first time in this document. Chemical composition of both the plants was determined by means of well-established chemical tests and modern spectroscopic techniques. Extracts were obtained from both the plants with the help of methanol and the extracts were tested for the measurement of antioxidant activity. In addition, antioxidant potentials were determined spectrophotometrically through the DPPH assay, Ferric ion (Fe+3) reducing antioxidant power assay, Ferric (Fe+3) reducing antioxidant potential (FRAP) assay and total antioxidant capacity (TAC) of the phosphomolybdenum assay. The potency of the DPPH assay of H. griffithii was 95.03% and C. leiocalycinus was 97.16% for the 1.0 mg/ml concentration. Moreover, the FRAP value of both the extract was 3.549mg of ascorbic acid/g of H. griffithii extract and 4.774mg of ascorbic acid/g of C. leiocalycinus extract. The total antioxidant capacity is a determination of water-soluble and fat-soluble antioxidants expressed a similar concentration dependent style (in mg/g ascorbic acid equivalent). H. griffithii and C. leiocalycinus have proved to be having high potent antioxidant activity.
Keywords: Antioxidant activity; DPPH; Extractions; FRAP; RP; Spectrophotometer; TAC