40. Identification of species-specific molecular markers in different farm animals by PCR-RFLP analysis

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Waqas Ahmad Khan Hamid Mustafa Umara Amir-u-Din, Maimoona Yousaf Adeela Ajmal Khalid Mehmood Muhammad Imran


Meat verification is relevant for public health and economic concerns. The present study identified species origin of raw meat samples of buffalo, cow, sheep, goat and chicken using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of a mitochondrial cytochrome b gene (359 bp). The amplified PCR products were digested using restriction enzymes Tas1 and Hinf1. DNA fragments of different lengths cleaved by two different enzymes were obtained. Each animal species had a distinctive combination of restriction fragments. PCR-RFLP analysis was performed by resolving digested products through agarose gel electrophoresis. Lengths of most of DNA fragments obtained for different farm animals were same as expected with few exceptions. PCR fragment obtained for buffalo remained uncut by enzyme HinfI. In short, PCR-RFLP method was successfully applied to detect the origin of meat species. This method is simple, quick and reliable for differentiation of meat species. The restriction patterns of cytochrome b are helpful in the discrimination of meat identification in a blind fashion.

Keywords: DNA; Enzymes; Meat; PCR-RFLP


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