Mutagenic effect of sodium azide (NaN3) on M2 generation of Brassica napus L. (variety Dunkled)

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Saddam Hussain, Wisal Muhammad Khan*, Muhammad Saleem Khan, Naveed Akhtar Nosheen Umar Sajjad Ali Sajjad Ahmed, Syed Sadaqat Shah

Abstract

The present study was aimed to investigate the effect of different doses (0.2%, 0.4%, 0.6% and 0.8%)  of sodium azide (SA) on  M2 generationof Brassica napus (variety Dunkled) on  some qualitative, quantitative and biochemical parameters. The result showed that days to germination, days to flowering and days to siliqua maturation were delayed (9.4, 98 and 153 days respectively) in higher treatment as compared to control (5.4, 96 and 147 days respectively). Higher concentration of SA (0.8%) decreased germination percentage (88.56%), plant height (97.95 cm), stem diameter (3.49 cm), number of branches/plant (3.49), number of leaves/plant (10), number of siliqua/plant (64.68), number of seed/siliqua (20.09) and 1000 seeds weight (3.91g) as compared to control. An increase was noticed for siliqua length (5.42 cm) in higher concentration of SA (0.8%) as compared to control (5.24 cm). Proximate analysis showed a significant decrease in oil, oleic acid and glucosinolates contents percentage (42.6%, 54.9%, and 58.5 μmol/g respectively) in higher concentration of SA (0.8%)  as compared to control (44.7%, 58.1% and 71.3 μmol/g respectively) while a significant increase was noticed in proteins, moisture, linoleic acid and erucic acid percentage (28.4%, 5.9%, 9% and 37.3% respectively) in higher concentration of SA (0.8%) as compared to control (26.8%, 5.5%, 8.4% and 35.2% respectively.


Keywords: Sodium Azide (SA); Brassica napus; morphological and biochemical parameters


http://dx.doi.org/10.19045/bspab.2017.60018

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