35. Evaluation of antimicrobial activity and ethnobotanical study of Cannabis sativa L.

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Musfirah Anjum, Zel-E-Arooj, Sidra Azam Pakeeza Rehman and Jamila Khadim


Plants are a great source of antibacterial components. Many plant extracts are being used in daily life to fight fungal and bacterial infections. In the present study in vitro, test confirmed the activity of antimicrobial of Cannabis sativa L. extracts against different bacterial and fungal strains. Antimicrobial efficiency of C. sativa a therapeutic plant (extracts of leaf) was screened using acetone, chloroform, water and ethanol, as solvents and tested against three Bacteria: E. coli, Staphylococcus aureus and P. aeruginosa, Fungi: A.niger and Fusarium spp. by disk diffusion and agar well diffusion method. All the plant extracts exhibited significant effects on antimicrobes, but the ethanolic extract and acetone extracts of C. sativa exposed highest zone of inhibition against most of the microorganisms that are under study. The minimum zone of inhibition was determined in distilled water and chloroform extract of C. sativaexhibited  minimum activity of antimicrobial against some the tested strains. To find out the ethno botanical uses of C. sativa a survey from the rural people of study area (Bhimber Rajani) was carried out, by interviewing the local inhabitants through a questionnaire. It was noticed that it is used assedative, narcotic, intoxicant and analgesic. As a green manure leaves are used. Hashish or Charas is also prepared from it. The powder is used for bronchitis, cough and chest problems.

Keywords: Antibacterial activity; Ethnobotany; Medicinal plants


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