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Shabi Creek receives saline water from the west bay of Gwadar and fresh water from Ankara River and its associated watershed. In Shabi Creek only Avicennia Marina is found out of three reported species of mangrove in Pakistan. Although Pakistan’s mangroves are widespread, and harbors very diverse biota has received less attention in terms of their microbial diversity particularly yeast. Marine yeasts are considered as a food source for some marine invertebrates and zooplankton and have been reported to produce useful compounds of human concern. In this study, we investigated the yeast diversity along the mangrove ecosystem of Shabi Creek, Gwadar. Yeast strains were extracted from different sections of mangrove plant such as root, stem, bark, branches, flowers, leaves, seawater, sediments, associated organisms and plants. Total 605 colonies were isolated which were further identified by their morphology and biochemical analysis and 17 distinct colonies were picked and were further analyzed by 26S rDNA gene amplicon. Sequence analysis was carried out with the help of CLC sequence viewer and the phylogenetic tree was constructed. In total 05 species of yeast were identified belonging to 04 genera i.e. Candida, Debaryomyces, Saccharomyces and Schizosaccharomyces. The identified species were Candida parapsilosis, Debaryomyces hansenii, Debaryomyces fabryii, Saccharomyces cerevisiae, Saccharomyces bayanus, Shizosaccharomyces pombe. The most dynamic growth of yeasts was observed in marine sediments among all samples. To the best of our knowledge, this is the first ever study conducted to investigate the biodiversity of mangrove ecosystem of Makran coast Gwadar, Pakistan.
Keywords: Gwadar; Mangrove ecosystem; Marine yeast diversity; Shabi Creek