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The study was carried out to examine the incidence of liverfluke infestation and pathological examination in sheep in Mirpur Azad Jammu &Kashmir. Abattoir and butchers shops were regularly monitored and 200 animals were examined before slaughter and 200 blood and liver samples were collected for biochemical, gross and histopathological study. On the basis of age of animals they were divided into young and adult age group having 100 animals in each group.A subjective evaluation of the gross lesions were performed based on the enlargement of liver, pale discoloration, friability, hemorrhages, discovery of extent of flukes, bile duct obstruction and cholangitis. The cumulative score of gross lesions in adult sheep was quite higher as compared to young sheep.Study has revealed that there was increase in the serum level of glucose, urea, creatinine, triglycerides, ALT, AST, ALP and GGT in adult group of sheep whereas there was apparent decrease of protein in adult sheep. However, in sheep of young age group all the serum biochemical parameters are unaffected. This study has showed that biochemical profile including blood glucose, liver enzymes (AST, ALT, ALP, GGT), urea and creatinine were elevated appreciably (P<0.05) and there was significantly decrease of total protein (P<0.05) in infected adult sheep in comparison with young age sheep. Microscopic changes revealed in the study have demonstrated structural changes in parenchyma and billiary tract. Histopathologically, atrophy, death of fatty tissues, multiple areas of death of hepatocytes, inflammation of liver, bile duct fibrosis and death of parenchyma were also examined in some cases of chronic fasciolosis.
Keywords: Biochemical; Fascioliasis; Gross pathology; Necrosis; Seroepidemiology