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This study is revealed on pasteurized Hot-fill orange juice the sequel of ascorbic acid, processed water and head space for 90 days during storage at room temperature. Outcomes were exhibited the significant reduction of the Alicyclobacillus growth with the addition of an amount of 150mg/lit of vitamin C and head space finishing of thermosetting container. While, initial bacterial count was controlled by ozonated and ultraviolet light processed water. Savour and color impermeability in the pasteurized Hot-fill orange juice were significantly (p < 0.05) pompous by both addition of vitamin C and leaving no head space. While in contrast the non- significant effect on acidity, pH, 0Brix, reducing and non-reducing sugar were observed during the storage. Hence, concluded that during processing as well as in storage the microbial growth especially Alicyclobacillus could be inhibited by processed water, leaving no head space and ascorbic acid fortification.
Keywords: Pasteurized orange juice; Processed water; Sensory analysis; Vitamin C