27. Evaluation of antibacterial and antioxidant activity of Aloe vera (Aloe barbadensis Miller) gel powder using different solvents
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Abstract
The present study was planned to investigate the phytochemical compounds present in Aloe vera and antimicrobial and antioxidant activity of Aloe vera gel powder with different solvents. The phytochemical compounds were screened qualitatively and glycosides, flavonoids, tannins, phenols, saponins, proteins, triterpeniods gave positive results while alkaloids and phlobatannin gave negative results. The polyphenol and flavonoid contents and antioxidant activity of Aloe vera gel powder extract using three different solvents (ethanol, methanol, acetone) were analyzed. The ethanolic extract showed maximum polyphenol (28.44 ± 2.67 mg GAE/g), flavonoid (63.90± 2.39 mg CE/g) and DPPH radical scavenging activity (51.09±0.33%). Antibacterial activity of extracts was examined by agar diffusion method with four microbial species (Salmonella typhi, Escherchia coli, Bacillus subtilis and Staphylococcus aureus). Maximum antibacterial activity was observed in ethanol extract of Aloe vera gel powder. Hence, Aloe vera gel powder can be used as an antimicrobial and antioxidant agent in food preservation and to prevent diseases related to oxidative stress.
Keywords: Aloe vera gel powder; Antibacterial activity; Antioxidant capacity; Phytochemicals; Solvents; Total polyphenols