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An in vitro propagation protocol was developed for Sesame (Sesamum indicum L. cv.SVPR - 1). In the present study the most important aspects of callus induction (CI) in vitro and regeneration of sesame seedlings (Sesamum indicum L.) were investigated. The explants were grown in basal medium Murashige and Skoog (MS) supplemented with different concentrations ratios of auxins and cytokines for the in vitro CI and the regeneration of plantlet. The callus commencement and induction was distinguished within 8 and 15 days after inoculation. Among the all combinations, 2, 4-dichlorophenoxyacetic acid (2, 4-D) and BAP with their respective concentrations of 2.0 and 0.5 ppm were more effective for IC frequencies in all explants. The maximum number and maximum length of shoots (11.8 ± 0.7, 9.2 ± 0.6 cm), the maximum number and maximum length of roots (6.2 ± 0.7, 6.6 ± 0.8 cm) were well demonstrated for MS with 2.0 ppm Kinetin (Kin) and 2 ppm indole-3-butyric acid in the explant of callus derived from the root. Seedlings with 3 to 5 shoots were transferred to pots of mixtures with equal amount of sand, soil and perlite for acclimation of field conditions and further multiplication.
Keywords: Callus induction; In vitro; Mass propagation; Sesame (Sesamum indicum L.)