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A proficient and speedy in vitro regeneration is a prerequisite for genetic modification of plants. The described protocol presents an efficient and practicable procedure for regeneration and multiple shoots induction by using shoot tips as explants for cucumber (Cucumis sativus L.). First, aseptic seedlings were established and shoot tips were excised from those seedlings. The cucumber seedlings used after 7 days of germination were found to be the best source of explants. Theses shoot tips were placed on MS medium supplemented with different concentrations of Benzyl amino purine (BAP) (0.5-2.5mg/L). It was observed that MS medium augmented with 1.5mg/L BAP resulted in maximum shoots formation. By increasing BAP beyond 1.5mg/L, rate of shoots formation was decreased. For rooting purpose, Indole Butyric Acid (IBA) (0.5-2.5mg/L) was used. Shoots greater than 2cm in size were shifted to rooting medium. Best roots development took place on MS medium having 1mg/L IBA. The plantlets with well-developed roots were shifted to peat moss containing pots and gradually acclimated to soil. This in vitro regeneration protocol can be used for genetic transformation of cucumber in future.
Keywords: BAP; Cucumber; IBA; In vitro regeneration; Shoot tips