83. Comparative profiling of gossypol enantiomers and seed traits’ related transcripts in cotton lines

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Aron Michael Felts, Deependra Bhatta, Sarabjit Bhatti, Ahmad Naseer Aziz


Cotton (Gossypium spp.) is the world’s third most important oilseed crop as well as an economic source of protein. However, the presence of (-)- gossypol limits the nutritional value of cottonseeds.  To genetically profile relevant traits, seeds of the two cotton parental lines TM-1 (G. hirstum) and 3-79 (G. barbadense) along with their 17 chromosomal substitution (CS) progenies were quantified for (-)- and (+)- enantiomer levels.  The TM-1 parent was found to contain a higher ratio of (+)-:(-)- gossypol enantiomers (13.34:8.52 µg/mg) compared to that of 3-79 levels (6.69:7.41 µg/mg) in the seeds.  Among progeny CS-B (containing substituted chromosomes from G. barbadense in G. hirsutum background) lines, only CS-B17 was found to contain significantly lower (7.65 µg/mg) level of (+)-enantiomer in its seeds, and also had the lowest (5.09 µg/mg) amount of (-)- gossypol among all lines.  A singular protocol was then devised for RNA yields from polysaccharide-rich cottonseeds of CS-B17 and its parents.  Transcription derived fragments (TDFs) analyses of these three lines were conducted on seeds harvested at 28, 35 and 42 days post anthesis (DPA).  64 TDFs profiles were analyzed using differential expression analyses, and similarities were revealed between 3-79 and CS-B17 along with unique expression trends for CS-B17.  Such expression analyses, which are based on single chromosome differences with TM-1 parent while reflecting seed nutritional merit of 3-79 parent, may facilitate cotton breeding endeavors.

Keywords: Chromosome substituted lines; Cotton; High performance liquid chromatography; Nutrition traits; RNA extraction; Transcription derived fragments


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